Fig. 4. Fatostatin inhibits growth of and induces EnRS in MCF-7 xenograft tumors.
MCF-7 cell xenograft tumors were initiated in athymic mice supplemented with estradiol capsules. Once tumors reached ~25 mm2, FS or DMSO control were administered daily (n = 12–14 tumors/group). a Tumor size was measured and plotted over time. b Tumors were excised after 16 days of treatment and weighed. Animal body weight on day 16 is indicated. c–e Ki67 and cleaved PARP were examined in FFPE tumor sections by IHC and quantified. Bars represent 100 µm. f p-eIF2α was examined by IF with DAPI as a nuclear stain in DMSO and FS-treated tumors. g Quantitation of immunofluorescence was performed using the cell seed/spot segmentation analysis in ImageJ FIJI. The number of cells with color intensity +5% over background were counted as positive staining for p-eIF2α. h The mean color intensity of each cell staining positive for p-eIF2α was determined and plotted as number of cells vs. intensity. i SREBP1 was examined by IF with DAPI as a nuclear stain in DMSO and FS-treated tumors. j The number of nuclei with SREBP1 staining was determined and plotted per 100 cells. * P < 0.05 vs DMSO control; ** P < 0.01 vs DMSO control