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. 2018 Aug 23;8:12659. doi: 10.1038/s41598-018-31079-0

Table 2.

Primer nucleotide sequences used in qPCR.

Genes accession number
(Gene Bank)
Forward and Reverse Primer Sequences Fragment size (bp)
Aa-β-actin EU531837 F: 5′-CCCCTGCTATGTATGTTGCCA-3′ 202
R: 5′-CGCTCGGTAAGGATCTTCATCA-3′
Aa-CPR EF197890 F: 5′-CGGAACAGCCATCTTATTCTTCG-3′ 149
R: 5′-GTTGCACGTACTCCTTAGTGG-3′
Aa-ECS AJ001539 F: 5′-GCAACAAGCCTACGAATCACTCAA-3′ 126
R: 5′-CGTGAAAAATTAAGGACCCTCATAGC-3
Aa-CPS AF472361 F: 5′-GAGGCGACATATTTGAGAGTGC-3′ 116
R: 5′-GATAGTGTTGGGTTGGTGTGA-3′
Aa-GAS DQ447636 F: 5′-CAAAGTGGTGGAAGGATATGAGGT-3′ 202
R: 5′-AGGCGAATCTCTTCAATGGTAGC-3′
Aa-BFS AY835398 F: 5′-CAAGGAGGAACAAGAGAGAGG-3′ 176
R: 5′-GCATAAGTAGAGGAAATGGGACA-3′
Aa-SQS AY445505 F: 5′-TGAGGTTTTCAGGGGTGTAGTC-3′ 166
R: 5′-CCTAGTGATGGTCGTTTGGGCA-3′
Aa-ADS HQ315833 F: 5′-CCGAGCAAGAAAGAAAACATAG-3′ 203
R: 5′-AACTTCAAGAAACTGGCACA-3′
Aa-ORA JQ797708 F: 5′-GGCGAGATTATGGCTTGGTACG-3′ 184
R: 5′-CGATGGTTGATGTGGTTCTTGTG-3′
Aa-ERF1 JN162091 F: 5′-TGAACTTCCCACATAGAATCGG-3′ 148
R: 5′-TCAACTACCTCAGCCAATGATAC-3′
Aa-WIRKY FJ390842 F: 5′-CAAGAACTACCAAGACCGAATCC-3′ 210
R: 5′-GGAGATAACAGGTGGCGAATAGAC-3′
Aa-ABCG6 Aan.67737 F: 5′-CGATAGCCAATAGCCATAAGTG-3′ 195
R: 5′-ATCCTACATTGCTTTCCATACG-3′
Aa-ABCG7 Aan.68336 F: 5′-GGTATCTGTAAATGGGGCAAAGTC-3′ 173
R: 5′-ACAATGGCATCCTCAACAACAC-3′