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. 2018 Aug 17;9:1897. doi: 10.3389/fmicb.2018.01897

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Copyright © 2018 Winter, Born and Pfeifer.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Western analysis of the various L/M transformants. Twenty micrograms of total protein were separated by SDS-PAGE, transferred to PVDF membranes and incubated with the antiserum raised against GvpM or GvpL. The second antibody was labeled with the fluorescence dye IRDye 800 CW (LI-COR) for detection. All blots are inverted to black and white. (A) Transformants carrying M/L-N/CGFP fusions and detection of GvpM using a GvpM antiserum. Arrows mark the M-NGFP and M-CGFP fusion proteins. (B) The same transformants as in (A) analyzed with the GvpL antiserum. The size of the L-NGFP fusions is marked by an arrow. (C) Detection of M_C and of M(mut)_C deletion variants in L/M transformants using the GvpM antiserum. (D) Detection of _NL in the same transformants using the GvpL antiserum. The expected protein size is marked by an arrow.