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. 2018 Aug 17;9:1897. doi: 10.3389/fmicb.2018.01897

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Copyright © 2018 Winter, Born and Pfeifer.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Deletion variants of GvpM and their Vac phenotype. (A) The 84-aa sequence of GvpM is given on top including the secondary structural elements α1, β1, β2, α2, and α3 shaded in gray. The different deletion variants are shown underneath. Dots refer to identical amino acids. The Vac phenotype observed with the respective Hfx. volcanii transformants is given on the right. Negative, gas vesicles were not observed; few GV, a single gas vesicle was found in a few cells, whereas other cells were Vac⁻; Vac⁺, fully gas-vesiculated cells. The fragments of GvpM used for the split-GFP analysis are indicated on top. (B) Transmission electron micrographs of various ΔM+MΔmut transformants. The size of the deletions at the N- or C-terminus of GvpM are indicated on top. Δ5N, Δ10N, and Δ10C were already described by Tavlaridou et al. (2014).