Skip to main content
. 2018 Aug 17;9:1946. doi: 10.3389/fmicb.2018.01946

FIGURE 3.

FIGURE 3

Cytotoxic activity of B. cereus culture supernatants in the presence of different milk products. Results of WST-1 bioassays performed on CaCo-2 cells are shown as reciprocal titers. In these experiments, B. cereus supernatants were applied as serial dilutions, while foodstuffs were added in constant concentrations as stated in Table 1. (A) Reference strain F837/76 (nhe+, hbl+) with foods. (B) Cytotoxicity of strain F837/76 under addition of different follow-on formula ingredients. The positive control differs from that in A, as it was obtained in an independent approach. (C) nheABC deletion mutant of F837/76 (hbl+). (D) Cytotoxicity of the nheABC deletion strain under addition of milk proteins. The positive control differs from that in (C), as it was obtained in an independent approach. (E) Reference strain NVH 0075-95 (nhe+). ∗Significant difference (P-value < 0.05). Data were statistically analyzed using the column statistics program (unpaired t-test, 95% confidence interval) of Graph Pad Prism version 5.00 (GraphPad Software, La Jolla, CA, United States).