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. 2018 Jul 19;218(7):1130–1141. doi: 10.1093/infdis/jiy228

Figure 6.

Figure 6.

Monocytes from patients with visceral leishmaniasis (VL) have reduced respiratory burst after interaction with Leishmania donovani parasites. A, Representative data showing mean fluorescent intensities (MFIs) of p47phox and p67phox within CD14+ cells from patients with VL and healthy endemic controls (ECs), measured by flow cytometry following culture for 3 hours in medium alone or with promastigotes or phorbol myristate acetate (PMA). Data are from 3 patients with VL and 8 ECs. B, L. donovani promastigotes induce reduced reactive oxygen species (ROS) production in CD14+ cells from patients with VL. Human monocytes were loaded with the CellROX probe for 1 hour following exposure to promastigotes. Representative contour plots (top right panel) and histograms (top left panel) of ROS expression are shown. Plots show ROS expression by CD14+ cells and ROS MFIs within CD14+ cells. Results are from 14 patients with VL before treatment and 11 ECs and are representative of 3 independently performed experiments.