Figure 7.

Interaction between CIB2 variants and α7B_M peptide monitored by fluorescence spectroscopy. (A) Trp fluorescence emission spectra of 4 μM α7B_M peptide alone (solid line) and incubated with of 8 μM CIB2 WT) (dotted black line) in the presence of 1 mM Mg²⁺ and 1 mM ${\text{Ca}}_{.}^{2+}$ (B) Trp fluorescence emission spectra in 1 mM Mg²⁺ and 1 mM Ca²⁺of 4 μM Scrb peptide alone (solid black line) and in the presence of 2 μM CIB2 WT (dotted red line), 4 μM CIB2 WT (short dashed blue line) and 8 μM CIB2 WT (dash dotted green line). (C) Fluorescence titration of 4 μM α7B_M peptide with WT (black circles) and E64D (red triangles) CIB2 in the presence of 1 mM Mg²⁺ and 1 mM Ca²⁺.