Fig. 2.

Identification of viruses infected in mosquito cultured cell line C6/36. A: Schematics of Shinobi tetravirus (SHTV) and Menghai rhabdovirus (MERV) genomic sequences. Encoded putative genes and their expected sizes are presented (RdRp: RNA-dependent RNA polymerase, VPg: Viral protein genome-linked, CP: capsid protein, N: nucleoprotein, P: phosphoprotein, M: matrix protein, G: glycoprotein, and L: RNA-dependent RNA polymerase). B: Phylogenetic analysis of SHTV and other viruses containing Permutotetraviridae. The amino acid sequences of putative RdRp were aligned and then analyzed using a maximum-likelihood method. Bootstrap replications are shown on the branches. C: Phylogenetic analysis of MERV and other rhabdoviruses. The amino acid sequences of L proteins were analyzed as described. D: Detection of SHTV or MERV in C6/36 JCRB strain and ECACC strain. The viral genome was determined by RT-PCR with the template RNA extracted from cell culture supernatant or cultured cells. PCR amplification from genomic DNA extracted from cultured cells were also determined. J: C6/36 JCRB strain, E: C6/36 ECACC strain. The original image is shown in Supplementary Material 1.