Table 1.
Sequences of primer sets with their corresponding PCR protocols and product sizes
| Target gene | Primer sequence (5′-3′) | PCR-protocol | Amplicon size | Reference |
|---|---|---|---|---|
| femA | F: TGCCTTTACAGATAGCATGCCA | 1 | 142 bp | [52] |
| R: AGTAAGTAAGCAAGCTGCAATGACC | ||||
| mecA | F: GGCTATCGTGTCACAATCGTT | 2 | 689 bp | [53] |
| R: TCACCTTGTCCGTAACCTGA | ||||
| spa | F: AGACGATCCTTCGGTGAGC | 3 | Variable | [54] |
| R: GCTTTTGCAATGTCATTTACTG | ||||
| lukM | F: TGAGTGGGTATGGCATGAAAGA | 4 | 572 bp | [20] |
| R: TGGACATTTTGTGTTACACCCC | ||||
| lukF’ | F: ACTCAGGCTATACCAACCCA | 1 | 425 bp | [20] |
| R: CGAGCTACTCTGTCTGCCAC | ||||
| pvl | F: GCTGGACAAAACTTCTTGGAATAT | 5 | 85 bp | [55] |
| R: GATAGGACACCAATAAATTCTGGATTG | ||||
| tst | F: CAACATACTAGCGAAGGAACT | 6 | 277 bp | [56] |
| R: GATATGTGGATCCGTCATTCA |
1 95 °C for 2 min, 35× (95 °C for 30 s, 59.5 °C for 30 s, 72 °C for 35 s), 72 °C for 5 min and 12 °C for ∞
2 95 °C for 1.5 min, 30× (95 °C for 45 s, 55 °C for 30 s, 72 °C for 45 s), 72 °C for 5 min and 12 °C for ∞
3 95 °C for 2 min, 30× (95 °C for 45 s, 60 °C for 45 s, 72 °C for 1:30 min), 72 °C for 5 min and 12 °C for ∞
4 95 °C for 2 min, 33× (95 °C for 30 s, 58.2 °C for 30 s, 72 °C for 30 s), 72 °C for 5 min and 12 °C for ∞
5 95 °C for 2 min, 35× (95 °C for 30 s, 55.5 °C for 30 s, 72 °C for 35 s), 72 °C for 5 min and 12 °C for ∞
6 95 °C for 2 min, 35× (95 °C for 30 s, 59 °C for 30 s, 72 °C for 30 s), 72 °C for 5 min and 12 °C for ∞