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. 2018 Jul 30;3:92. [Version 1] doi: 10.12688/wellcomeopenres.14703.1

Figure 3. The filter set ‘DV Elite QUADmC mCherry’ has been selected and then ‘Customise Filters’ button pressed to reveal the exact configuration.

Figure 3.

It can be seen that the excitation path has one dichroic in reflection mode, DV-SSI-Quad-mCh. Because a dichroic is part of both the emission and excitation light paths, it also appears in the emission path in transmission mode, note the blue R button in the Excitation Path and T button in the emission Path. In addition, the emission path includes the DV-EM-mCherry bandpass filter in transmission mode. The transmission or reflection mode of filters and dichroics can be toggled by clicking on the ‘T|R’ slider, or they can be removed from a path by clicking on the X button. New filters or dichroics can be added by dragging from the Filters list on the left into the the relevant Path box.