Figure 4.

Evaluation of IFNγ-producing ST2⁺ILC, CD56⁺, and CD3⁺ T cells in human PBMC. IFN-γ-producing cell subsets were evaluated using flow cytometry (A–C). Raw dot plots representative of IFN-γ⁺CD56⁺ cells from 8 asthmatic and 6 healthy donors. PBMCs from people with allergic asthma and non-allergic healthy controls were pre-exposed to media or IL-33 for 6 h and then cultured in the absence or presence of RV for 5 days (D). Abbreviations: PBMC, peripheral blood mononuclear cells; IL-33, interleukin 33; RV, rhinovirus 16; ST2⁺ILCs, ST2⁺ innate lymphoid cells. *p ≤ 0.05 and **p ≤ 0.01.