Skip to main content
. 2018 Aug 14;243(11):926–933. doi: 10.1177/1535370218788520

Figure 3.

Figure 3.

Nrf2 mediates Hcy-inhibited glycerol release in fully differentiated 3T3-L1 adipocytes. (a) Fully differentiated 3T3-L1 adipocytes were transfected with Nrf2 siRNA. Fully differentiated 3T3-L1 adipocytes were transfected with siRNA for Nrf2 and treated with 100 μmol/L Hcy. (b, c and d) Transfection of siRNA for Nrf2 into 3T3-L1 adipocytes decreased the protein and mRNA expression levels of Nrf2. (e and f) The cells and medium were harvested for TG and glycerol assays. (g and h) Cells were incubated with EGCG (100 μmol/L) or t-BHQ (50 μmol/L) for 24 h, the expression level of Nrf2 protein was increased. (i and j) The levels of TG. (k and l) Measurement of glycerol assay. Data are means ± SEM (n ≥ 3). *P < 0.05 compared with NC or control. NC: non-targeted control siRNA; Hcy: homocysteine; EGCG: epigallocatechin gallate; t-BHQ: tert-butylhydroquinone.