Fig. 3.

Corticotropin-releasing factor (CRF) receptor expression in monoaminergic cell body regions was measured from adult male rats (PND 60) using methods described by Lukkes et al. (2009a). Rats were exposed to either adolescent social defeat or control handling conditions from PND 35–39, as detailed by Watt et al. (2009) – a paradigm known to increase cocaine and amphetamine responses in adulthood (Burke et al., 2011, 2013, 2016; Burke and Miczek, 2015). A) Representative Western blot images of CRF₂ receptor, CRF₁ receptor, and actin (loading control) expression in the dorsal raphe nucleus (dRN). B) CRF₂, but not CRF₁ receptor expression was increased in the dRN of adult male rats exposed to adolescent social defeat. C) No changes in either CRF₁ or CRF₂ receptor expression was observed in the ventral tegmental area (VTA) or locus coeruleus (LC) in adult male rats exposed to adolescent social defeat. #P < 0.05 compared to controls. All data represented as mean values + S.E.M. N = 20 per group.