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Determination of Binding Selectivity
(A) FAM-labeled SW-4 was incubated with 786-O, HEK293T, and HK-2 cells. The binding ability was analyzed by flow cytometry. (B) 786-O, HEK293T, and HK-2 cells were incubated with Cy5-labeled SW-4 and library at 4°C. Fluorescence, bright-field, or merged confocal images are shown.
