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. 2018 Aug 4;12:758–768. doi: 10.1016/j.omtn.2018.07.015

Figure 4.

Figure 4

Internalization of SW-4b into 786-O Cells

(A) 786-O cells were incubated with Cy5-labeled SW-4b at 4°C or 37°C. Fluorescence image, bright-field, or merged confocal images are shown. (B) 786-O cells were incubated with Cy5-labeled SW-4b at 37°C. The two-dimensional and three-dimensional images were analyzed by confocal microscopy. (C and D) After 786-O cells were treated with cytochalasin D, amiloride, M-β-CD, or chlorpromazine, binding of SW-4b to 786-O was analyzed by flow cytometry (C) or confocal microscopy (D). (E) The colocalization of the Cy5-SW-4b (red) with caveolin 1 (green) is shown by confocal microscopy.