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. Author manuscript; available in PMC: 2018 Aug 24.
Published in final edited form as: Chem Res Toxicol. 2018 Jun 18;31(7):570–584. doi: 10.1021/acs.chemrestox.8b00005

Figure 3.

Figure 3

Relative P450 enzyme contribution to sunitinib metabolism. (A) M1, (B) M3, and (C) M5. Sunitinib (10 μM) was incubated with a panel of recombinant human P450 Supersomes (20 nM) in the presence of an NADPH regenerating system and supplemented with GSH (5 mM) for 10 min. Sunitinib metabolites were analyzed by LC–MS/MS analysis utilizing MRM. The MRM transitions for the metabolites indicated were as follows: M1(m/z 371 > 283), M3 (m/z 397 > 281), M5 (m/z 702 > 586). The MRM peak areas are shown for each metabolite. The results are shown as the means ± SD from four independent experiments (n = 4) performed in triplicate each.