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. 2018 Aug 23;10:111. doi: 10.1186/s13148-018-0542-8

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 8 — Estrogen deprivation does not affect neither ID4 methylation nor ID4 expression. a Left, ddPCR assay for the detection of ID4-methylated status in control and fulvestrant-treated MCF-7 cells. Results are presented as copies per microliter in the amplification reaction. Right, bar graph represents the mean of three technical experiments measuring the methylated/unmethylated ratios of the control and experimental conditions. b ID4 probe (A_23_P59375) intensity was compared between control and fulvestrant-treated MCF-7 cells