Table 1. The list of primer sequences and expected PCR product sizes .
Exons | Primers | Annealing temperature (°C) | PCR product length |
6 |
F: TGCCCTGCTTGAGACACCTA R: TCCTCCCCCAACTTTCTGC |
60 | 283 |
7 |
F: CCTCTGACTCAGTGGTGA R: CTTGCAGCAGGAAAAG |
59 | 256 |
8 |
F: CTGAGTCTGGCTTGGCTTAA R: CTCATTTGAGAAATTCAGGT |
58 | 194 |
11 |
F: TGCAGCAGGGAATACTGA R: AGATGAGTGGCACCAGT |
60 | 260 |
12 |
F: TCCAAATGGTGCCCT R: GGCGATGGTAGGGAA |
57 | 232 |