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. 2018 Aug 20;14:2105–2114. doi: 10.2147/NDT.S163449

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© 2018 Zhang et al. This work is published and licensed by Dove Medical Press Limited

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(A) TH immunostaining of section from the rat’s VTA treated with the saline (Control), 20% ethanol (Ethanol), 30 mg/kg ketamine (K30), 60 mg/kg ketamine (K60), 30 mg/kg ketamine with 20% ethanol (K30+E), and 60 mg/kg ketamine with 20% ethanol (K60+E), N = 3 per group, TH positive neurons (brown, arrows) were found in the VTA as shown, 200×. (B) AOD of immunohistochemical staining of TH calculated by Image Pro Plus 6.0 software. *Indicates p<0.05 compared to the saline control.

Abbreviations: AOD, average optical density; TH, tyrosine hydroxylase; VTA, ventral tegmental area.