Figure 2.

Extracellular ATP-induced resistance against the necrotrophic fungus B. cinerea. Wild-type, dorn1-3, and OxP2K1 plants were infiltrated with ATP (500 μM) and/or MeJA (10 μM) followed by inoculation with B. cinerea (5 × 10⁵ spores/mL). Different letters denote statistically significant differences based on analysis performed using the mixed model and Tukey honest test (P < 0.05). A, Pictures show infected leaves of wild type, dorn1-3, and OxP2K1 at 2-d postinoculation. Graph shows the lesion diameters induced by B. cinerea infection. Data are shown as means ± sd of three biological replicates (n = 10 leaves per treatment). B, Graph shows the fold change in the numbers of in planta-formed spores of B. cinerea 3-d postinoculation. Data are shown as means ± sd of three biological replicates (n = 15 leaves per treatment). C, Quantification of B. cinerea by real-time PCR was performed with DNA extracted from infected leaves of wild type (WT), dorn1-3, and OxP2K1. The data show expression values of B. cinerea gene BcPG relative to Arabidopsis reference gene AtSAND as means ± sd of three biological replicates (n = 6 leaves per treatment).