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. Author manuscript; available in PMC: 2018 Aug 24.
Published in final edited form as: J Immunol. 2006 Nov 1;177(9):5852–5860. doi: 10.4049/jimmunol.177.9.5852

Figure 3. Presence of Foxp3-positive cells in the mucosa and intestinal lymphoid follicles of patients with IBD.

Figure 3

Colon samples from normal controls and patients with ulcerative colitis (UC), Crohn’s disease (CD), non-IBD inflammations of the colon (diverticulitis (D), pseudomembranous colitis (PC), and CMV-induced colitis (CMV)) were analysed for CD3, CD4, and Foxp3. Sections from tonsil were analysed for CD4 and Foxp3. (a) Co-staining for CD4 and Foxp3 on human tonsil. Overview (×100) and high-power magnification. (b) CD3 and Foxp3 staining on colon tissue. (c) Density of CD3+ and Foxp3+ cells within colon. Numbers of cells were quantified per area at ×400 magnification. Each data point represents one patient. (d) Density of Foxp3+ cells within colonic LP, as well as T and B cell areas of mucosa- associated lymphoid tissue. Each data point represents one patient. (e) Co-staining of Foxp3 and IL-10 on human appendix tissue. Left, Co-staining of Foxp3, IL-10, and DAPI showing IL-10-positive cells within the germinal center (GC) and subepithelial dome (SED) close to the epithelium (E). Detail of Foxp3+IL-10+ cells within the subepithelial dome area are also shown. Right, Co-staining of Foxp3, IL-10, and CD3. Detail shows IL-10+CD3+Foxp3+ cells as well CD3+Foxp3+ cells that are in close contact with IL- 10+CD3Foxp3 cells.