Figure 5. Accumulation of the IL-10-producing progeny of CD4⁺CD25⁺ T cells within the colon.

Rag-1^−/− mice were injected with 4 × 10⁵ wild-type CD45.2⁺CD4⁺CD45RB^high T cells. After development of colitis, mice received a second transfer of 1 × 10⁶ congenic CD45.1⁺ CD4⁺CD25⁺ T cells. After 4 wk, the ability of CD4⁺CD45RB^high (n = 6) and CD4⁺CD25⁺ (n = 6) progeny to produce IL-10 and IFN-γ was analysed. Lymphocytes were prepared from spleen, MLN, and the colonic LP and stimulated with PMA/ionomycin and brefeldin A. The results of two independent experiments are pooled. (a) Representative FACS plot showing preferential IL-10 production by LP CD4⁺CD25⁺ T cell progeny (CD45.1⁺) and preferential IFN-γ production by the CD4⁺CD45RB^high T cell progeny (CD45.1⁻). (b) Production of IL-10 and IFN-γ by CD4⁺CD25⁺ T cell progeny and comparison of IL-10 and IFN-γ production by CD4⁺CD45RB^high T cell progeny in the presence or absence of CD4⁺CD25⁺ T cells. Data are shown for spleen, MLN, and LP. Significance was tested using the Mann-Whitney U test; n.s., not significant.