Fig 2. Transcriptional, cAMP-concentration dependent, and reversible response of MdESF to decidualizing stimuli.

(A) GO categories of down-regulated genes in MdESF after treatment with decidualizing stimuli for 2 days relative to unstimulated control (n = 3, p < 10⁻⁶). Terms related to cell cycle are indicated in red. (B) Concentration-dependent morphological response of MdESF to 8-br-cAMP/MPA treatment after 6 hours (top panels) and 22 hours (bottom panels). (C) MdESF morphological response to 8-br-cAMP/MPA treatment is reversible after 1 day in growth media. Images were taken 2 hours after treatment, at which time media with decidualizing stimuli were changed to growth media. Images were subsequently acquired after 19 hours in growth media. Scale bars are 10 μm. Underlying data for GO analysis are provided in S1 Data. cAMP, cyclic AMP; GO, gene ontology; MPA, medroxyprogesterone acetate