KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit anti-histone H2A | Abcam | Cat#ab13923; RRID: AB_300750 |
| Rabbit anti-Phospho-p70 S6 Kinase (Thr389) (108D2) | Cell Signaling Technology | Cat#9234S; RRID: AB_2269803 |
| Rabbit anti-Phospho-S6 Ribosomal Protein (Ser235/236) (91B2) | Cell Signaling Technology | Cat#4857S; RRID: AB_2181035 |
| Rabbit anti-4E-BP1 | Cell Signaling Technology | Cat#9452; RRID: AB_331692 |
| Rabbit anti-HIF-1α (D2U3T) | Cell Signaling Technology | Cat#14179S; RRID: AB_2622225 |
| Rabbit anti-Phospho-H2AX (Ser139) (20E3) | Cell Signaling Technology | Cat#9718; RRID: AB_2118009 |
| Rabbit anti-Phospho-AMPKα (Thr172) (40H9) | Cell Signaling Technology | Cat#2535; RRID: AB_331250 |
| Rabbit anti-DYKDDDDK Tag Antibody | Cell Signaling Technology | Cat#2368; RRID: AB_2217020 |
| Rabbit anti-β-Tubulin (9F3) | Cell Signaling Technology | Cat#2128S; RRID: AB_823664 |
| Rabbit anti-β-Actin (13E5) | Cell Signaling Technology | Cat#4970S; RRID: AB_2223172 |
| Horse anti-mouse horseradish peroxidase-conjugated | Cell Signaling Technology | Cat#7076; RRID: AB_330924 |
| Goat anti-rabbit horseradish peroxidase-conjugated | Cell Signaling Technology | Cat#7074; RRID: AB_2099233 |
| Mouse anti-GST | Cell Signaling Technology | Cat#2624; RRID: AB_2189875 |
| Mouse anti-Myc (9E10) | Harlan Laboratories | Cat#200613 |
| Mouse anti-8-Hydroxydeoxyguanosine | Millipore | Cat#MAB3560; RRID: AB_94925 |
| Rabbit anti-phospho histone H2A (Ser129) | Millipore | Cat#07-745-I; RRID: AB_492642 |
| Rabbit anti-SOD2 | Millipore | Cat#06-984; RRID: AB_310325 |
| Mouse anti-Penta-His | Qiagen | Cat#34660; RRID: AB_2619735 |
| Rabbit anti-SOD1 | Santa Cruz | Cat#sc-17767; RRID: AB_628301 |
| Rabbit anti-goat horseradish peroxidase-conjugated | Thermo-Fisher | Cat#A16142; RRID: AB_2534813 |
| Goat anti-mouse Alexa Fluor 594-conjugated | Thermo-Fisher | Cat#A11005; RRID: AB_141372 |
| Goat anti-mouse Alexa Fluor 488-conjugated | Thermo-Fisher | Cat#A11001; RRID: AB_2534069 |
| Goat anti-rabbit Alexa Fluor 594-conjugated | Thermo-Fisher | Cat#R37117; RRID: AB_2556545 |
| Goat anti-rabbit Alexa Fluor 546-conjugated | Thermo-Fisher | Cat#A11010; RRID: AB_2534077 |
| Rabbit anti-TAP tag | Thermo-Fisher | Cat#CAB1001; RRID: AB_10709700 |
| Bacterial and Virus Strains | ||
| E. coli DH5α | Transgen | Cat#CD201 |
| E. coli BL21 (DE3) | Transgen | Cat#CD601 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Rapamycin | LC Laboratories | Cat#NC9362949 |
| Dihydrorhodamine 123 | Thermo-Fisher | Cat#D632 |
| Dihydroethidium | Thermo-Fisher | Cat#D11347 |
| TEMPOL | Sigma-Aldrich | Cat#176141 |
| Nitroblue tetrazolium | Sigma-Aldrich | Cat#N6876 |
| Anti-FLAG M2 affinity gel | Sigma-Aldrich | Cat#F2426 |
| Glucose-free DMEM | Thermo-Fisher | Cat#11966025 |
| Lipofectamine™ 3000 | Thermo-Fisher | Cat#L3000015 |
| Glutathione Agarose | Thermo-Fisher | Cat#16100 |
| Critical Commercial Assays | ||
| Lipid Peroxidation (MDA) Assay Kit | Abcam | Cat#ab118970 |
| Universal Kinase Assay Kit | Abcam | Cat#ab138879 |
| QIAexpress Ni-NTA Fast start Kit | Qiagen | Cat#30600 |
| Deposited Data | ||
| Original data | Mendeley data | http://dx.doi.org/10.17632/rvbvczzd5d.1 |
| Experimental Models: Cell Lines | ||
| Human: Hep3B | ATCC | Cat#HB-8064 |
| Human: Hep3B (SOD1-GFPWT) | This study | N/A |
| Human: Hep3B (SOD1-GFPT40A) | This study | N/A |
| Human: Hep3B (SOD1-GFPT40E) | This study | N/A |
| Human: A549 | ATCC | Cat#CCL-185 |
| Human: A549 (SOD1-GFPWT) | This study | N/A |
| Human: A549 (SOD1-GFPT40A) | This study | N/A |
| Human: A549 (SOD1-GFPT40E) | This study | N/A |
| Human: HEK293FT | Thermo-Fisher | Cat#R70007 |
| Human: HEK293T | ATCC | Cat#CRL-3216 |
| Human: MCF7 | ATCC | Cat#HTB-22 |
| Experimental Models: Organisms/Strains | ||
| Mouse: BALB/c female nude mice | Beijing Vital River Laboratory Animal Technology | Stock#: 11400700232586 |
| S. cerevisiae MATa hisΔ1 leu2Δ0 met15Δ0 ura3Δ0 | Tsang et al., 2014 | FM391/S288C |
| S. cerevisiae MATa hisΔ1 leu2Δ0 met15Δ0 ura3Δ0 tor1 Δ::KanMX pYDF80-TOR1S1792I | Li et al., 2006 | SZy 997 |
| S. cerevisiae MATa hisΔ1 leu2Δ0 met15Δ0 ura3Δ0 sod1Δ::KanMX pRS415 | This study | SZy 1050 |
| S. cerevisiae MATa hisΔ1 leu2Δ0 met15Δ0 ura3Δ0 sod1Δ::KanMX pRS415(SOD1WT-MYC9) | This study | SZy 1051 |
| S. cerevisiae MATa hisΔ1 leu2Δ0 met15Δ0 ura3Δ0 sod1Δ::KanMX pRS415(SOD1S39A-MYC9) | This study | SZy 2542 |
| S. cerevisiae MATa hisΔ1 leu2Δ0 met15Δ0 ura3Δ0 sod1Δ::KanMX pRS415(SOD1S39D-MYC9) | This study | SZy 2543 |
| S. cerevisiae MATa hisΔ1 leu2Δ0 met15Δ0 ura3Δ0 sod1Δ::KanMX pRS415(SOD1S39E-MYC9) | This study | SZy 2544 |
| S. cerevisiae MATa hisΔ1 leu2Δ0 met15Δ0 ura3Δ0 sod1Δ::KanMX | Dharmacon | 16913 |
| S. cerevisiae MATa hisΔ1 leu2Δ0 met15Δ0 ura3Δ0 sod2Δ::KanMX | Dharmacon | 16605 |
| S. cerevisiae MATa hisΔ1 leu2Δ0 met15Δ0 ura3Δ0 ccs1Δ::KanMX | Dharmacon | 10614 |
| S. cerevisiae MATa hisΔ1 leu2Δ0 met15Δ0 ura3Δ0 pRS415 | This study | SZy 2550 |
| S. cerevisiae MATa ura3-1 leu2-3,-112 his3-11,-15 trp1-1 ade2-1 can1-100 tor1 Δ::KanMX tor2-dg | This study | SZy 2551 |
| S. cerevisiae MATa hisΔ1 leu2Δ0 met15Δ0 ura3Δ0 SOD1-TAP::HIS3 | This study | SZy 2552 |
| S. cerevisiae MATa ura3-1 leu2-3,-112 his3-11,-15 trp1-1 ade2-1 can1-100 | Tsang et al., 2014 | W303 |
| S. cerevisiae MATa ura3-1 leu2-3,-112 his3-11,-15 trp1-1 ade2-1 can1-100 MAF1-MYC9::TRP1 | This study | SZy 1701 |
| S. cerevisiae MATa ura3-1 leu2-3,-112 his3-11,-15 trp1-1 ade2-1 can1-100 MAF1-MYC9::TRP1 pRS426(SOD1-MYC9) | This study | SZy 3000 |
| S. cerevisiae MATa ura3-1 leu2-3,-112 his3-11,-15 trp1-1 ade2-1 can1-100 MAF1-MYC9::TRP1 pRS426(SOD1S39A-MYC9) | This study | SZy 3001 |
| S. cerevisiae MATa ura3-1 leu2-3,-112 his3-11,-15 trp1-1 ade2-1 can1-100 MAF1-MYC9::TRP1 pRS426(SOD1S39E-MYC9) | This study | SZy 3002 |
| S. cerevisiae MATa ura3-1 leu2-3,-112 his3-11,-15 trp1-1 ade2-1 can1-100 MAF1-MYC9::TRP1 pRS426(SOD1S39D-MYC9) | This study | SZy 3003 |
| S. cerevisiae MATa ura3-1 leu2-3,-112 his3-11,-15 trp1-1 ade2-1 can1-100 MAF1-MYC9::TRP1 pRS426 | This study | SZy 3004 |
| S. cerevisiae MATa hisΔ1 leu2Δ0 met15Δ0 ura3Δ0 sod1Δ::KanMX CCS1-TAP::His1 pRS415(SOD1WT-MYC9) | This study | SZy3005 |
| S. cerevisiae MATa hisΔ1 leu2Δ0 met15Δ0 ura3Δ0 sod1Δ::KanMX CCS1-TAP::His1 pRS415(SOD1S39A-MYC9) | This study | SZy3006 |
| S. cerevisiae MATa hisΔ1 leu2Δ0 met15Δ0 ura3Δ0 sod1Δ::KanMX CCS1-TAP::His1 pRS415(SOD1S39E-MYC9) | This study | SZy3007 |
| Oligonucleotides | ||
| Primers for S39A mutagenesis: Forward: CGAGATCGCTGGTAACGCTCCTAACGCAGAACGTG Reverse: CACGTTCTGCGTTAGGAGCGTTACCAGCGATCTGC | This study | N/A |
| Primers for S39D mutagenesis: Forward: TTACGAGATCGCTGGTAACGATCCTAACGCAGAACGTGG Reverse: CCACGTTCTGCGTTAGGATCGTTACCAGCGATCTCGTAA | This study | N/A |
| Primers for S39E mutagenesis: Forward: CTTACGAGATCGCTGGTAACGAGCCTAACGCAGAACGTGGGTT Reverse: AACCCACGTTCTGCGTTAGGCTCGTTACCAGCGATCTCGTAAG | This study | N/A |
| Primers for GST-SOD1 construct: Forward: CCGGAATTCATGGTTCAAGCAGTCGCAGT Reverse: CCGCTCGAGGTTGGTTAGACCAATGACACCAC | This study | N/A |
| Primers for T40A mutagenesis: Forward: TCCATGCAGGCCTTCAGCCAGTCCTTTAATGCTTC-3 Reverse: GAAGCATTAAAGGACTGGCTGAAGGCCTGCATGGA | This study | N/A |
| Primers for T40E mutagenesis: Forward: TGGAATCCATGCAGGCCTTCCTCCAGTCCTTTAATGCTTCCC C Reverse: GGGGAAGCATTAAAGGACTGGAGGAAGGCCTGCATGGATTCCA | This study | N/A |
| Primers for gap repair: Forward: CTCTTCGCTATTACGCCAGC Reverse: ATTAATGCAGCTGGCACGAC | This study | N/A |
| Primers for construction of CCS1-TAP strains Forward: TGA ACC ACC CAG AAA ACG AG Reverse: AGG ATT GGA AAC CGG CTT TG | This study | N/A |
| Recombinant DNA | ||
| FLAG-mTORWT | This study | pSZ92 |
| FLAG-mTORD2338E (Kinase Dead) | This study | pSZ96 |
| HIS6-hSOD1WT | This study | pSZ1366 |
| HIS6-hSOD1T40A | This study | pSZ1366-b |
| GST-4EBP1 | This study | pSZ1130 |
| hSOD1WT-GFP | Stevens et al., 2010 | Addgene plasmid, Cat#26407 |
| hSOD1T40A-GFP | This study | pSZ1458 |
| hSOD1T40E-GFP | This study | pSZ1459 |
| hSOD1WT-FLAG | Somwar et al., 2011 | N/A |
| hSOD1T40A-FLAG | This study | pSZ1460 |
| hSOD1T40E-FLAG | This study | pSZ1461 |
| SOD1WT-MYC9 | This study | pSZ374 |
| SOD1S39A-MYC9 | This study | pSZ1398a |
| SOD1S39D-MYC9 | This study | pSZ1398b |
| SOD1S39E-MYC9 | This study | pSZ1398c |
| GST-SOD1WT | This study | pSZ1410 |
| GST-SOD1S39A | This study | pSZ1410b |
| Software and Algorithms | ||
| Protein Data Bank | Hart et al., 1999 | 2JCW |
| Protein Data Bank | Banci et al., 1999 | 1DSW |
| Other | ||