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. 2017 Sep;58(11):4447–4456. doi: 10.1167/iovs.17-21600

Figure 1.

Figure 1

Increased cAMP content detected by radioimmunoassay in the epithelium of intact lenses exposed to hyposmotic solution. Lenses were incubated for 2, 5, or 10 minutes in control (isosmotic; 300 mOsm) or hyposmotic (200 mOsm) Krebs solution either in the absence or in the presence of 500 μM IBMX, a phosphodiesterase inhibitor added to prevent cAMP breakdown. Values are mean ± SEM of results from five to eight lenses. ** and *** indicate a significant difference (P < 0.01 and P < 0.001) from control in the absence of IBMX. # and ### indicate significant difference (P < 0.05 and P < 0.001) from the respective time-matched control in the presence of IBMX.