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. 2017 Sep;58(11):4447–4456. doi: 10.1167/iovs.17-21600

Figure 2.

Figure 2

The effect of PKA inhibitor H89 (10 μM) on the SFK phosphorylation and Na,K-ATPase activity responses to hyposmotic solution. Intact lenses were exposed to hyposmotic Krebs solution (200 mOsm) for 2 minutes in the presence or absence (control; Con) of H89 added 20 minutes beforehand. Then the epithelium was removed, homogenized, and subjected to Western blot analysis for SFK phosphorylation at Y416 or used to determine Na-K-ATPase activity. β actin was probed as a loading control in Western blot analysis. (A) A representative SFK Western blot (upper panel) and pooled data (lower panel) on phospho-SFK (pY416 SFK) band density (mean ± SEM of results from six independent experiments). (B) Na,K-ATPase activity as mean ± SEM of results from four to six lenses. ** or *** indicates a significant difference from control (P < 0.01 or P < 0.001), and ## indicates a significant difference from group subjected to hyposmotic solution alone (P < 0.01).