Figure 5.

The effect of PP2, a specific SFK inhibitor, on the SFK phosphorylation and Na,K-ATPase activity responses to hyposmotic solution (Hypo). Intact lenses were exposed to hyposmotic Krebs solution (200 mOsm) for 2 minutes in the presence or absence (Control) of 10 μM PP2, added 45 minutes beforehand. Then the epithelium was removed, homogenized, and subjected to Western blot analysis for SFK phosphorylation at Y416. Values are mean ± SEM of results from six independent experiments. ** indicates a significant difference (P < 0.001) from control.