Figure 8.

Expression of calcium-activated adenylyl cyclase proteins in porcine (A) and human (B) lens epithelium. Using Western blot, epithelium samples isolated from fresh porcine or human lenses were probed for ADCY1, ADCY3, or ADCY8. Pig liver (PL), pig kidney cortex (PK), or pig brain (PB) and human retina (H Ret), human kidney cells (HK2), or human retina were used as positive control for ADCY1, ADCY3, or ADCY8, respectively. One hundred micrograms of protein was loaded per lane for positive controls, and 150 μg protein was loaded for adenylyl cyclases. Note that ADCY3 and ADCY8 were present in both porcine and human lens epithelium. ADCY1 was not detectable in either species.