Figure S7.

Raphin1 Is Beneficial in HD^82Q Mice, Related to Figure 7

Note that the results shown here were obtained with a separate cohort than the ones shown in Figures 7B, 7D, and 7F.

(A) Total body weight gain of wild-type and HD^82Q mice treated orally with Raphin1 at 2 mg/kg or vehicle once a day for four weeks daily. Data are means ± SEM, n = 27, 26, 19, 21 for WT Vehicle, WT Raphin1, HD^82Q Vehicle and HD^82Q Raphin1, respectively. ^∗∗p < 0.01 by two-way ANOVA with Tukey’s multiple comparisons test.

(B) Quantifications of huntingtin assemblies from immunoblots such as (Figure 7C) performed on cortex lysates from 2.5-month-old mice following treatment with Raphin1 or vehicle from 4 weeks of age. Data are means ± SEM, n = 3. ^∗p < 0.05 by unpaired two-tailed Student t test.

(C) Quantifications of nuclear huntingtin inclusions (see STAR Methods) revealed with 2B4 antibody in the cortex of 2.5-month-old mice following treatment with Raphin1 or vehicle from 4 weeks of age. Data are means ± SEM, n = 7 (vehicle) and n = 10 (Raphin1). ^∗p < 0.05 by unpaired two-tailed Student t test.