REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Pierce High Sensitivity Streptavidin-HRP antibody	ThermoFisher Scientific	Cat#21130
Rabbit polyclonal e-IF2α-P antibody	ThermoFisher Scientific	Cat#44-728G; RRID: AB_2533736
Mouse monoclonal e-IF2α antibody	Abcam	Cat#ab5369; RRID: AB_304838
Mouse monoclonal tubulin antibody	Sigma-Aldrich	Cat#T5168; RRID: AB_477579
Mouse monoclonal BiP antibody	BD Biosciences PharMingen	Cat#610978; RRID: AB_398291
Rabbit polyclonal ATF4 antibody	Santa Cruz Biotechnology	Cat#sc-200; RRID: AB_2058752
Rabbit polyclonal Ppp1r15a antibody	Proteintech	Cat#10449-1-AP; RRID: AB_2168724
Rabbit polyclonal Ppp1r15b antibody	Proteintech	Cat# 14634-1-AP; RRID: AB_2300036
Mouse monoclonal CHOP antibody	ABR Affinity BioReagents	Cat#MA1-250; RRID: AB_2292611
Mouse monoclonal huntingtin antibody 2B4 antibody	Euromedex	Cat#HU-2B4
Mouse monoclonal huntingtin antibody 1C2 antibody	Euromedex	Cat#PQ-1C2
Goat polyclonal Alexa Fluor 488 antibody	ThermoFisher Scientific	Cat#A11001; RRID: AB_2534069
Mouse monoclonal MBP-HRP antibody	New England BioLabs	Cat#E8038; RRID: AB_1559738
Rabbit polyclonal e-IF2α antibody	Abcam	Cat#ab26197; RRID: AB_2096478
Rabbit polyclonal e-IF2α-P (Ser51) antibody	Cell Signaling Technology	Cat#9721; RRID: AB_330951
Bacterial and Virus Strains
E. coli BL21-GOLD (DE3) pLysS	Agilent Technologies	Cat#230134
E. coli BL21/pGro7	Takara	Cat#9122
Chemicals, Peptides, and Recombinant Proteins
MBP-R15A325-636-His (R15A)	Das et al., 2015	N/A
MBP-R15B340-698-His (R15B)	Das et al., 2015	N/A
bio-PP1c1-322 (bio-PP1c)	This study	N/A
his-PP1c1-322 (his-PP1c)	This study	N/A
MBP-R15A325-512R15B636-698-His (R15AN-R15BC)	Carrara et al., 2017	N/A
MBP-R15B340-635R15A513-636-His (R15BN-R15AC)	Carrara et al., 2017	N/A
PP1c7-330 (PP1c)	Carrara et al., 2017	N/A
GST-PERK	Carrara et al., 2017	N/A
eIF2a1-185-His	Carrara et al., 2017	N/A
bio-GBZ	Tsaytler et al., 2011	N/A
Guanabenz (GBZ)	Sigma-Aldrich	Cat#G110
Sephin1	Das et al., 2015	N/A
Raphin1	This study	N/A
Compound C3	This study	N/A
MG-132	Cell Signaling technology	Cat#2194
NSM-873	Selleckchem	Cat# S7285
CB-5083	Cayman Chemicals	Cat#2194
CalyculinA	Cell signaling technology	Cat#9902S
CellTox Green Dye, 1,000X	Promega	Cat#G873B
Cycloheximide	Sigma-Aldrich	Cat#C7698
Hematoxylin	VWR International	Cat#351945S
Oil Red O solution	VWR International	Cat#101410-976
RNeasy Mini Kit	QIAGEN	Cat#74104
iScript cDNA Synthesis Kit	Bio-Rad	Cat#1708891
SYBR Select Master Mix	Applied Biosystems	Cat#44-729-08
O.C.T. compound	VWR International	Cat#361603E
Hoechst 33342	Lonza	Cat#PA-3014
Critical Commercial Assays
Bac-to-Bac Baculovirus Expression System	ThermoFisher Scientific	Cat#10359-016
EnzChek Phosphatase Assay Kit	ThermoFisher Scientific	Cat#E12020
Accu-Chek Aviva Blood Glucose Meter	Roche	Cat#06351557018
Accu-Chek Aviva Glucose Test Strips	Roche	Cat#06453970
Experimental Models: Cell Lines
HeLa (Female)	Sigma-Aldrich	IGBMC, Illkirch, France
MEF cells: Ppp1r15a −/− (R15a −/−). Sex undetermined.	Harding et al., 2009	N/A
MEF cells: Ppp1r15b −/− (R15b −/−) Sex undetermined.	Harding et al., 2009	N/A
S. frugiperda Sf9 SFM adapted insect cells. Sex undetermined.	ThermoFisher Scientific	Cat#11496015
Experimental Models: Organisms/Strains
C57BL/6J	The Jackson Laboratory	Cat#000664
B6C3-Tg(HD82Gln)81Gschi/J (N171-82Q)	The Jackson Laboratory	Cat#003627
CMT-1B	Lawrence Wrabetz	N/A
B6.129P2-Ppp1r15atm1.1Ajf/Mmnc (R15a −/−)	MMRRC (mutant mouse resource and research centers)	Cat#30266
Oligonucleotides
R15a – f: GACCCCTCCAACTCTCCTTC	Sigma-Aldrich	N/A
R15a – r: TCTCAGGTCCTCCTTCCTCA	Sigma-Aldrich	N/A
Chop – f: GGAGAGAGTGTTCAAGAAGGAAGTG	Sigma-Aldrich	N/A
Chop – r: GCAGGTCCTCATACCAGGCTT	Sigma-Aldrich	N/A
Gapdh – f: TGGGTGGTCCAGGGTTTCTTACTCCTT	Sigma-Aldrich	N/A
Gapdh – r: CGACTTCAACAGCAACTCCCACTCTTCC	Sigma-Aldrich	N/A
Recombinant DNA
pMAL-c5x	New England BioLabs	Cat#8108S
Bac-to-Bac vector kit (pFastBac1)	ThermoFisher Scientific	Cat#10360014
pDW464	Addgene	Cat#8845
Plasmid pMAL-c5x-R15A325-636 (N-terminal MBP-tag, C-terminal 6 × His-tag)	Das et al., 2015	N/A
Plasmid pMAL-c5x-R15B340-698 (N-terminal MBP-tag, C-terminal 6 × His-tag)	Das et al., 2015	N/A
Plasmid pDW464-PP1γc1-322 (N-terminal BAP-tag)	This study	N/A
Plasmid pFastBac1-6 × His-PP1γc1-322 (N-terminal 6 × His-tag)	This study	N/A
Plasmid pMAL-c5x-R15A325-512R15B636-698 (N-terminal MBP-tag, C-terminal 6 × His-tag)	Carrara et al., 2017	N/A
Plasmid pMAL-c5x-R15B340-635R15A513-636 (N-terminal MBP-tag, C-terminal 6 × His-tag)	Carrara et al., 2017	N/A
Plasmid modified pGEX6p1-PP1αc7-330 (the vector's GST-tag was replaced by an N-terminal Thio6/His6-tag (MGSDKIHHHHHH)).	Carrara et al., 2017	N/A
Plasmid pGEX-4T-1-PERK537-1114 (N-terminal GST-tagged murine PERK kinase domain)	Addgene	Cat#21817
Solubility enhanced human eIF2a (amino acids 1-185) with a C-terminal His-tag	Ito et al., 2004	N/A
Software and Algorithms
Biacore T200 Evaluation Software	GE Healthcare	https://www.biacore.com/lifesciences/service/downloads/software_licenses/biaevaluation/
GraphPad Prism 7	GraphPad Software	https://www.graphpad.com/scientific-software/prism/
ImageJ	NIH	https://imagej.nih.gov/ij/
IncuCyte Zoom software	Essen BioScience	https://www.essenbioscience.com/en/
ViewPoint	ViewPoint Behavior Technology, France	http://www.viewpoint.fr/en/home
Coulbourn Instruments	Coulbourn Instruments, Allentown, PA, USA	https://www.coulbourn.com/
Graphic State software	Coulbourn Instruments, Allentown, PA, USA	https://www.coulbourn.com/
NIS Elements	Nikon Instruments	https://www.nikoninstruments.com/en_EU/Products/Software