Figure 1.

HSP70 overexpression antagonizes cisplatin-induced HGC-27 cell apoptosis. HGC-27 cells were transfected with GFP-HSP70 plasmid or control plasmid. After 24 h, cells were stimulated with 5 µg/ml cisplatin for the indicated times. (A) Nuclear morphology of apoptotic cells was detected by DAPI staining (magnification, ×100). (B) Expression levels of apoptosis-related proteins PARP, pro-caspase-3 and cleaved caspase-3 were detected by western blotting. (C) Apoptosis rate was determined by flow cytometry (representative plots and quantification is shown). ^**P<0.01, with comparisons indicated by lines. HSP70, heat shock protein 70; GFP, green fluorescent protein; PARP, poly-ADP-ribose-polymerase; DDP, cisplatin; CON, control.