Figure 3.
The formation of vessel-like structures of SHEDs. (A) SHEDs subjected to shear stress (4 and 16 dynes/cm2, 2 h) treatment were reseeded onto matrigel for in vitro angiogenesis assay. Vessel-like structures of SHEDs at 4, 8 and 12 h were detected using an inverted microscope (×4). (B) Comparison of tubule length between different shear stress value groups. (C) Comparison of branching points between different shear stress value groups. (D) SHEDs-EphrinB2-RNAi subjected to shear stress (4 and 16 dynes/cm2, 2 h) treatment were reseeded onto matrigel for in vitro angiogenesis assay. Vessel-like structures of SHEDs at 4, and 8 h were detected using an inverted microscope (×4). (E) Comparison of tubule length between different shear stress value groups. (F) Comparison of branching points between different shear stress value groups (*P<0.05).