Table II.
Screening the most effective sequence among four shRNA sequences against PCSK9 by quantitative reverse transcription-polymerase chain reaction.
| Group | Relative PCSK9 mRNA expression in kidney
|
|
|---|---|---|
| 24 h | 48 h | |
| NFD-C57BL/6 | 1 | 1 |
| HFD-C57BL/6 | 2.226 | 2.226 |
| HFD+PCSK9 shRNA control | 1.905 | 1.905 |
| HFD+PCSK9 shRNA sequence 1 | 1.717 | 1.625 |
| HFD+PCSK9 shRNA sequence 2 | 1.570 | 1.566 |
| HFD+PCSK9 shRNA sequence 3 | 1.357 | 1.304 |
| HFD+PCSK9 shRNA sequence 4 | 1.412 | 1.489 |
NFD, no-fat diet; HFD, high-fat diet; PCSK9, proprotein convertase subtilisin/kexin type 9; shRNA, short hairpin RNA.