Figure 3.

Localization of EphrinB2 and the effect of EphrinB2 expression without Ti and EphB4-Fc. (A) Detection of ephrin B2 gene expression by reverse transcription quantitative polymerase chain reaction in different treatment groups on days 1, 3 and 5. (B) EphrinB2 expression on the cell membrane surface visualized using immunofluorescence microscopy. (C) The protein expression level of the cell membrane ligand was detected by western blot analysis on day 3. Data are presented as mean ± standard deviation. ^*P<0.05 and ^**P<0.01. M-CSF, macrophage-colony stimulating factor; RANKL, receptor activator of nuclear factor κB ligand; ephB4, erythropoietin-producing human hepatocellular receptor 4; Ti, titanium.