(A) Blood ammonia levels of control and comatose FHF mice were measured (Control, n = 5; AOM, n = 4). Mean + SEM, *, P < 0.05 versus control mice by two-tailed Student’s t test. (B) Brains from control and comatose FHF mice were harvested after intravenous injection of EBD. Extravasated brain EBD was quantified fluorometrically (Control, n = 6; AOM, n = 5). Mean + SEM, *, P < 0.01 versus control mice by two-tailed Student’s t test. (C) Serum ATX activities in control and comatose FHF mice were measured (Control, n = 10; AOM, n = 12). Mean + SEM, *, P < 0.0001 versus control mice by two-tailed Student’s t test.