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. Author manuscript; available in PMC: 2019 Jul 2.
Published in final edited form as: Biochem Biophys Res Commun. 2018 May 24;501(4):1048–1054. doi: 10.1016/j.bbrc.2018.05.106

Fig. 3. LPA disrupted BBB barrier function in in vitro BBB model.

Fig. 3

(A) Schematic representation of the in vitro BBB model is shown. mRNA expression in three primary cultured cells were examined by RT-PCR. (B) Immunofluorescent staining of confluent RBECs in BBB model for tight junction proteins ZO-1, claudin-5 and occludin. Cells were immunostained 15 min after 50 μM LPA stimulation. Arrows show zipper-like staining between LPA-treated RBECs, while the junctional immunostaining of RBECs forms a continuous, smooth and belt-like pattern (arrowheads) in vehicle-treated RBECs (Bar, 10 μm). (C) Change of TEER in BBB model treated with 50 μM LPA and 200 ng/ml PTX was evaluated by an epithelial-volt-ohm meter. Data is representative of three independent experiments with similar results. (D) Change of TEER in BBB model was evaluated by an epithelial-volt-ohm meter. Cells were pretreated with or without 10 μM Y-27632 and 10 μM Ki16425 for 10 min and then stimulated with 50 μM. Data is representative of three independent experiments with similar results.