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. Author manuscript; available in PMC: 2019 Jul 2.
Published in final edited form as: Biochem Biophys Res Commun. 2018 May 24;501(4):1048–1054. doi: 10.1016/j.bbrc.2018.05.106

Fig. 4. LPA disrupted barrier function of RBEC monolayer cultures through LPA6.

Fig. 4

(A) Change of cell index in RBECs treated with 5, 10, 20 and 50 μM of LPA was evaluated by the xCELLigence system (n = 3). Data is representative of two independent experiments with similar results. Mean - SEM (B) RBECs were pretreated with or without 10 μM Y-27632 and 10 μM Ki16425 for 10 min. Then 10 μM LPA was added and the change of cell index was monitored (n = 3). Data are representative of two independent experiments with similar results. Mean - SEM. (C) mRNA expression profile of LPA receptors in RBECs pericytes and astrocytes were analyzed by RT-PCR. Arrowheads indicate the 200-bp DNA size marker. (D) Expression levels of LPA6 and β-actin mRNA are presented as a percentage of those with control siRNA (n = 2). Data is representative of three independent experiments with similar results. (E) Change of cell index in RBECs treated with 50 μM LPA was evaluated by xCELLigence system 48 h after siRNA transfection (n = 3). Data is representative of three independent experiments with similar results. Mean ± SEM.