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. Author manuscript; available in PMC: 2019 Sep 1.
Published in final edited form as: J Autoimmun. 2018 Jun 20;93:45–56. doi: 10.1016/j.jaut.2018.06.004

Figure 4. Antibiotic Treatment Worsens Dacryoadenitis in CD25KO mice.

Figure 4

Wild-type (WT) and CD25KO mice were subjected to a cocktail of oral antibiotics (ABX) for 4 weeks starting at 4 weeks of age and compared to CD25KO at 8 weeks of age that drank normal water (n = five/group). All groups were born and raised in conventional specific-free pathogen vivarium. (A) Representative pictures of haematoxylin and eosin (H&E)-stained sections of lacrimal glands. Large inset squares are high magnification of small insets, 10X objective, scale bar = 100 μm. (B) Inflammation scores of lacrimal gland pathology. Bar graphs show means ± SEM of two independent experiments, final n = five animals. Nonparametric Mann–Whitney U statistical tests were used to make comparisons.(C) Relative fold of expression of IFN-γ and IL-12 in lacrimal glands. Bar graphs show means ± SEM of five samples per group. Parametric t-tests were used to make comparisons between groups.

****P<0.0001 wild-type vs. CD25KO comparisons (with and without antibiotics)