Fig. 4.

Retention of GFP fluorescence. (a) Confocal imaging of cortical neurons in Thy1-GFP-M mouse brains before and after various methods clearing. Each image is a maximum projection of image stacks (40 to $60\mu \mathrm{m}$). Scale bar, $50\mu \mathrm{m}$. (b) The relative changes of mean intensity of fluorescence and total intensity of fluorescence after uDISCO, 3DISCO, SeeDB, ScaleS, and ScaleSQ(5), CUBIC, $Clea{r}^{T2}$, and PACT clearing (Error bars denote standard deviations; $n=15$, 14, 15, 13, 17, 17, 13, and 21 neurons, respectively).