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. 2018 Aug 24;9:3411. doi: 10.1038/s41467-018-05646-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — METTL13-mediated methylation in cells and tissues. a, b Individual assessment of the methylation status of eEF1A1 and eEF1A2 in human cells. FLAG-tagged eEF1A1 and eEF1A2 were overexpressed in HEK-293 cells and the methylation status of the N terminus (a) and Lys55 (b) was assessed by MS. c, d Assessment of the methylation status of eEF1A proteins in a panel of rat tissues. Same as in previous panels, but ion chromatograms represent the collective methylation status of both eEF1A1 and eEF1A2 in rat liver, kidney, and intestine. e, f Assessment of eEF1A methylation in HeLa cells stressed by various compounds. Ion chromatograms representing the methylation status of eEF1A in cells treated with anisomycin, cycloheximide, 4NQO, and AdOx are shown. Peaks corresponding to the mono- and dimethylated forms of the eEF1A N terminus are indicated (arrow). g, h Quantitative analysis of eEF1A methylation in HeLa cells treated with AdOx. Significance was assessed using a two-tailed t-test and error bars represent the s.d., n = 4