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. 2018 Aug 24;9:3411. doi: 10.1038/s41467-018-05646-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — METTL13 gene deletion affects translation. a Schematic of the experimental set-up of a ribosome foot-printing experiment. b The relative occupancy of mRNA codons in the ribosome acceptor site (A-site) in WT versus KO cells is shown (closed circles). As control, the codon occupancy values in the downstream codon (A-site + 1 codon) are shown (open circles) and the spread of this data is indicated (dashed lines). Symbol size represents codon frequency in quartiles (larger is more frequent). Error bars represent s.d., n = 3. c, d Quantitative assessment of key aminoacyl-tRNA synthetases and components of the eEF1 complex in WT and METTL13 KO cells. c The abundance (iBAC value) for the cytosolic aminoacyl-tRNA synthetases for Ala (AARS), Pro (EPRS), His (HARS), Lys (KARS), Asn (NARS), Arg (RARS), Ser (SARS), Thr (TARS), Trp (WARS), and Tyr (YARS) is shown. d The abundance of eEF1A1 and eEF1A2 as well as the remaining components of the eEF1 complex (eEF1B2, eEF1D, eEF1E1, and eEF1G) are shown. Error bars represent s.d., n = 3