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. 2018 Aug 24;8:12791. doi: 10.1038/s41598-018-31222-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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GFP CETN2 foci present a loose association with the PCM. (A) Transmitted light and fluorescent images of incompetent oocytes and follicular cells expressing GFP CETN2. GFP CETN2 signal (gray) is shown in follicular cells (upper panels) and incompetent oocyte (lower panels). Additional panels for the incompetent oocyte show the co-localization of GFP CETN2 (green) with mCherry-Plk4 (magenta). (B) GFP CETN2 foci (gray) moving from the cortex of the oocyte to the nuclear envelope in an incompetent oocyte. Insets show higher magnifications of the foci. (C) Oocyte followed at prophase I exit. GFP CETN2 appears gray. Insets show higher magnifications of the CETN2 foci. (D) Oocyte observed from prophase I exit to first polar body extrusion by transmitted light (upper panel) and fluorescence emission (lower panels). GFP CETN2 (gray) foci are highlighted with black arrows. Partial co-localization with mCherry-Plk4 (magenta) is shown. (A–C) GFP CETN2-expressing cells from transgenic mice, injected with mCherry-Plk4 cRNA (A). (D) mCherry-Plk4-expressing oocytes from transgenic mice, injected with Venus CETN2 cRNA. Time is expressed in hours and minutes.