Fig. 2.

JunB cKOs display impaired skin homeostasis. a Representative photomicrographs with immunostaining of FABP5 (red), indicative of sebaceous glands, in dorsal back skin from wild type and JunB cKO mice. Nuclei stained with DAPI in blue. Scale bars, 50 µm. b Histology of tail skin from wild type and JunB cKO mice. Scale bars, 50 µm. c Representative photo of colony-forming unit assay from FACS purified HFSCs (CD34^+veα6Itg^Hi) from wild type and JunB cKO mice back skin that were cultured in vitro for 2 weeks and then stained with Rhodamine B and Nile blue. n = 3 mice per genotype. d FACS analyses from second telogen phase displaying an increased ratio of P2 to P1 hair follicle stem cells (CD34^+veα6Itg^Hi /CD34^+veα6Itg^Low) in unperturbed dorsal skin from 60 days old JunB cKO compared to wild type mice. e Confocal images of whole mount tail epidermis depict differentiated keratinocytes immunostained for the terminal differentiation marker k10 (green) in JunB cKO compared to wild type skin. Nuclei stained with DAPI in blue. Scale bars, 50 µm. E, epidermis; SG, sebaceous gland