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. 2001 Nov 6;98(24):13625–13630. doi: 10.1073/pnas.241516898

Figure 1.

Figure 1

Effect of EGTA on ryanodine-modified Ca2+ release channels. Single-channel currents are shown as downward (A) or upward (B) inflections from the closed level at −30 mV or +30 mV, indicated by a dotted line in each trace. Tracings were recorded in symmetrical 250 mM KCl solution. The upper traces in both A and B were recorded in the absence of EGTA to give a pCa of 6.5, and the lower traces were recorded in the presence of 1 mM EGTA, which lowered the Ca2+ concentration to a pCa of 10. Modulation of the channel by ryanodine is shown in the upper trace at −30 mV (A). The ryanodine-modified channel had about half the conductance of the normal channel. The Po shown above each trace is for the ryanodine-modified channel. Traces were filtered at 1 kHz and digitized at 10 kHz. The averaged Po for the ryanodine-modified channels measured in A and B before and after the addition of 1 mM EGTA is shown in C.