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. 2018 Aug 24;8:12815. doi: 10.1038/s41598-018-31153-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Evaluation of lipid extraction (a) Images of DiI-stained tissue at different ETC time points. (b) Pre-labeled CM-DiI signals remaining in the brain vasculature before (left) and after 2 hr ETC (right). Lipid extraction rate (c) and transparency (d) at different ETC time points. “X” marked points in (d) indicate an optimal time point for each organ for maximal clearing. N = 3, 3, 6, 6 for brain, lung, liver and kidney, respectively. (e) Correlation graph of extracted lipid and contribution of lipid extraction to the improvement of the transparency of each organ.