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. 2018 Aug 24;8:12815. doi: 10.1038/s41598-018-31153-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Molecular diffusion-based evaluation method for tissue clearing (a) Conceptual image showing the evaluation process of tissue clearing with two variables: transparency and time constant (τ) for the diffusion of RI-matching solution. (b) Time-transparency data fitted to one-phase association function to get time constant and final transparency. (c) τ profile of each organ at different ETC time points. (d) Sample images of the same mouse kidney slice: bright-field image (left), transparency map (middle), τ map (right). (e) τ-transparency scatter plot, which could indicate the optical properties of a cleared tissue. Each data points were obtained from 3 independent samples in each organ at each ETC time point. (f) τ and transparency relations for each points of a kidney sample before (red dots) and after 12 hr ETC (blue dots) and their position change (colored arrows). The colors of arrow were from τ values before ETC. (g) Images of mouse kidney at ETC 0 and 12 hr. (h) Scatter plot of τ-transparency from samples in (g).