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. 2018 Aug 24;8:12749. doi: 10.1038/s41598-018-31158-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Relevance of individual tRNA modifications for ochre suppression by SUP4. (a) Schematic representation of SUP4 tRNA. Modifications are indicated according to³⁶ together with proteins essential for their formation. (b) Red pigmentation of yeast strains of the indicated genetic background as an indicator for ade2-1 suppression. Strains were streaked on YPD, grown for 4 days, followed by incubation at 4 °C for an additional 4 days prior to documentation. (c) Plate assays of ade2-1 and can1-100 suppression by SUP4. Strains of the indicated genetic backgrounds were spotted on YPD, −Ade or −Arg/+Can media and photographed after 40 h of incubation at 30 °C (YPD and −Arg/+Can) or at the indicated time points (−Ade).