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. 2018 Aug 24;8:12749. doi: 10.1038/s41598-018-31158-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Additive SUP4 defect in ncl1 tef1 mutants and SUP4 rescue efficiency of other modification mutants by overexpression of TEF1. (a) Plate assays for ade2-1 (−Ade) and can1-100 (−Arg/+Can) readthrough in ncl1 tef1 mutants compared to respective single mutants. (b) Plate assay for ade2-1 readthrough in indicated tRNA modification mutants carrying either vector (YEplac181) or overexpression construct for TEF1 (h.c. eEFA, pTEF1). Plates were photographed at indicated time points. YPD plate in (B) was photographed after 48 h.