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. 2018 Aug 24;8:12749. doi: 10.1038/s41598-018-31158-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Heat sensitivity and growth defects in single and double tRNA modification mutants in the presence and absence of TEF1 overexpression. h.c. eEF1A refers to overexpression of TEF1 (pTEF1). Vector refers to YEplac181. Cells were taken from selective YNB medium and dilutions prepared in sterile water. After spotting of cell dilutions on YPD plates, these were incubated at 30 °C, 37 °C or 39 °C as indicated. Plates were photographed at the indicated time points.