Figure 2.

Reaction of synthetic nitroalkenes with nucleophiles in different environments. (A) Spectral change upon reaction of 10 µM NATOH with 0.1 mM βME in TMA20 buffer at pH 8.1 containing 2.5 g/L SDS. (B) First-order plots obtained at different concentrations of βME measuring absorbance at 350 nm. (C) Second-order plot for the same experiment, linear fit parameters k_f (slope) = 2.3 M⁻¹ s⁻¹, k_r (intercept) = 5 × 10⁻³ s⁻¹. (D) Spectral change upon titration of NATOH in CHAPS micelles. The arrows indicate the change upon increase in pH of the bulk solution. (E) Titration curves of NATOH at 350 nm in TMA20 buffer and different micelle suspensions, from left to right, CTAB, CHAPS, Triton X-100 and SDS. The absorbance was normalized to the acidic and alkaline extrapolated values of the fit. (F) Apparent pK_a results of NATOH and NATxME in different micellar media.